In silico restriction map and PFGE


Restriction Enzyme: BsmBI,Esp3I
Recognition sequence: CGTCTCN'NNNN_
Genera: Mycoplasma

Results are not available when the number of fragments is over 50.
Place cursor over bands to show the genome.




 
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(24)


(94)


































(32)
Compute Dice distance and UPGMA clustering
When comparing band patterns in gels, one alternative is: 1) to assign value 1 to the presence of bands and to assign value 0 to their absence; 2) to compare data from each sample (to compute Dice distances); and 3) to perform UPGMA clustering. By clicking below, the process above will be applied.

Be aware that the comparison performed by this service is theoretical and it allows discerning bands that are just one base different. In contrast, those tiny differences cannot be detected in wet lab experiments.


Show distances
Show cluster as text

Compute Pearson distance and UPGMA clustering
When comparing bands patterns in gels, one alternative is: 1) to determinate an array of data for each line composed of the position in the gel (distance) and color in that position; 2) to compare the previous arrays (to compute Pearson's distances); and 3) to perform UPGMA clustering. By clicking below, the process above will be applied.

Be aware that the comparison performed by this service is theoretical and it allows discerning bands that are just one base different. In contrast, those tiny differences cannot be detected in wet lab experiments.


Show distances
Show cluster as text


Show bands
1234567891011121314151617181920212223242526272829303132333435363738394041424344454647484950515253545556575859606162636465
(32)
88471
88020
74565
62625
58979
58892
58494
56755
47195
39741
39118
37126
35270
34802
30689
26359
25152
24214
20355
19106
18042
14151
12860
6259
6226
5299
4926
4703
3208
3208
1793
99
(39)
89142
73054
68635
63322
54465
48863
48674
33633
33205
30111
29529
27263
26630
25945
25267
24993
20489
19058
18843
16554
14782
13881
9407
7797
7078
7022
6972
6253
6226
5302
3208
2901
2668
2408
1794
1538
307
150
69
(40)
105457
67491
58731
57892
39804
38712
33544
30265
30190
28957
25643
24993
24382
23760
20938
20460
19983
19761
15798
13859
13717
12233
10797
10349
9494
8613
8455
7670
4889
4860
4839
4464
4177
4106
3569
3245
1686
1577
829
264
(41)
93048
79359
62262
58513
52910
51903
50643
49432
46931
42933
38477
35329
33848
33283
28485
28411
21999
21978
16941
15337
14423
13861
13178
12049
9889
9221
9068
6743
6583
6325
5180
4984
3444
3207
3205
2384
2360
2007
1131
369
69
(39)
90974
77775
58515
52559
51906
49431
48423
46936
43146
39886
35329
33849
33281
30847
28486
28257
23529
21978
16941
15337
14423
13784
13178
12049
9890
9068
6998
6745
6584
6325
5180
4984
3208
2384
2360
2007
1131
369
69
(54)
NA
(20)
145276
131071
122929
94930
71268
65437
62860
49716
46548
42163
32623
25818
22587
19721
19466
16973
13448
11769
10779
4641
(29)
103467
93037
92408
88321
64508
48082
43399
37802
36328
27623
27362
25398
19347
18347
15041
14961
12882
11477
10894
9936
9850
9007
6524
6261
4476
4269
3109
1566
532
(47)
82149
79317
70188
66669
59780
55656
42931
38875
31144
30052
28632
27589
25337
24540
24185
22044
19628
18139
15283
13333
13155
12428
12058
11459
11158
11063
10937
10059
9925
8822
8215
7076
6661
5784
5148
3019
2831
2736
2055
1347
1297
567
463
463
71
63
48
(23)
123713
122357
108193
76272
73962
60398
56679
52918
40258
38625
37745
34042
24810
22284
19520
19080
16701
15268
15201
14782
12487
6915
5913
(28)
138128
116960
96823
90862
71151
49101
45236
43375
39965
39676
35630
33800
33548
21055
17773
15733
15283
12335
11121
10930
9394
8462
7226
6078
2920
2628
1767
564
(32)
137062
113301
91088
83856
82010
75563
73404
51294
43125
37031
36949
35052
33799
33548
22031
21054
20649
17758
17091
12915
12335
11121
10924
9392
8462
7227
6753
6079
2920
2627
1767
564
(26)
136948
135200
127767
77987
76359
63474
57377
43118
37031
33799
33548
22029
21055
20646
17758
17085
14926
12903
11382
11121
10924
9392
7227
2627
1767
564
(35)
98699
96175
68763
62332
61986
60543
51327
49707
48183
40079
38458
35658
34625
26913
24053
23738
22530
21480
16900
16321
13111
12993
11284
8607
6419
5257
4210
4163
2938
2816
2742
1473
1277
348
304
(35)
96181
69566
68765
62332
61985
60511
51324
49707
48180
40111
38458
36008
34382
28702
26400
24030
22560
21473
16900
13104
13002
11284
8607
6419
5257
4209
4161
3272
2938
2816
2742
1473
1358
348
304
(34)
96153
69566
68766
62323
62059
60519
51321
49707
48190
41482
40093
38458
36007
34351
24027
23812
21479
16900
13104
12960
11284
8607
6419
5257
4209
4161
3535
2938
2816
2742
1473
1280
348
304
(36)
96109
69556
68765
67359
62332
60491
51323
49707
48181
40114
38459
35657
34646
26876
24032
24031
22518
21472
17444
16900
13111
12981
11293
8607
6419
5257
4209
4161
2938
2816
2742
1473
1280
348
304
78
(35)
98654
96169
68765
67349
62332
60519
53366
51323
48175
40108
38460
35663
34692
26900
24050
24031
22536
21477
16900
16848
13112
12981
11293
8607
6419
5257
4209
4161
2938
2816
2742
1473
1280
348
304
(36)
96197
69554
68764
67340
62332
60511
51324
49707
48183
43599
40111
38458
35652
34636
26776
24089
22528
21479
17244
16888
13110
11293
8607
6419
5257
4852
4209
4160
2938
2816
2742
1473
1277
348
348
304
(33)
111120
84021
82466
68768
62552
62487
58384
54115
49831
40582
38463
36792
30836
24119
21481
20545
19430
19161
18241
17479
15068
12139
11300
8000
6419
5629
5257
4208
2816
2742
1319
348
304
(39)
137685
94134
79063
72096
62754
40274
39169
38168
34244
33116
30826
27607
25167
24720
24029
21478
21382
20610
16894
15080
14780
14733
13719
12302
11301
9202
9075
7495
6963
6420
5257
4158
3813
2816
1514
1474
1274
348
304
(35)
96187
78077
69563
68764
62332
60492
51323
49707
48182
40135
38459
35657
34662
26786
24047
24031
22519
21479
16900
16848
13111
13002
11293
8607
6419
5257
4209
4161
2938
2816
2742
1473
1280
348
304
(32)
96176
72163
69564
68765
62314
61986
60487
51323
49729
48564
48182
38458
35656
24027
23765
21478
17444
16900
13111
11293
8607
8245
6419
5257
4209
4161
2938
2816
2742
1473
1288
304
(28)
101977
95848
89620
80344
80242
56855
49811
48326
39264
38466
38058
35123
30650
27301
23895
21551
21482
20648
17046
15082
11873
11284
6419
5632
5257
2742
1521
1295
(33)
111084
84020
82450
68768
62531
62474
58406
54106
49824
40582
38463
36793
33568
30831
24083
22068
21481
20543
18228
17425
15065
12139
11300
7997
6419
5629
5257
4208
2816
1498
1319
348
304
(5)
221117
147249
133878
64558
13274
(5)
220997
147076
133869
64342
13274
(5)
221107
147201
133860
64537
13272
(5)
220961
146918
133858
64497
13270
(5)
221058
147095
133865
64506
13272
(106)
NA
(171)
NA
(161)
NA
(22)
91296
68960
59542
51453
49286
47332
40311
39039
30820
29157
26775
26415
24578
16661
16392
14320
12398
9056
6530
2631
1656
837
(27)
125328
117581
75783
64217
54670
51301
50426
44187
42441
37533
35173
31521
26852
25834
21019
20908
19475
17516
13918
12826
9226
8245
7819
5440
4781
1429
127
(25)
121473
120834
90680
75726
62284
54675
51298
49091
44190
37513
31519
26852
25833
21030
20922
19478
17516
12827
9226
8245
8107
5437
4781
1429
127
(28)
109513
104281
83159
60977
60667
54702
50761
48893
43118
42174
37559
31522
24535
21365
17657
15643
15328
12826
11574
9224
8228
7794
5973
5861
4781
2683
1833
127
(29)
129383
109072
75359
62270
53900
53814
48855
46280
44237
31550
26195
23631
20336
20236
19448
19283
17713
15637
13935
12828
11601
9246
8252
7797
5861
4781
4502
2366
127
(30)
134262
107892
74384
62299
57510
56753
50773
48112
35851
35145
31596
29957
23302
20338
19432
19362
16663
15638
13931
12836
9228
8353
8254
7797
5861
5449
4781
2363
1830
127
(22)
178484
106918
88609
77033
67850
56861
54943
47543
40193
31503
22087
21737
21331
20328
15585
12829
11618
8239
6267
5484
1836
127
(18)
151254
83046
77802
75111
65864
65238
58461
54181
42404
38338
38139
34488
20522
20156
6575
4312
1464
92
(18)
151254
83046
77818
75111
65881
65238
58461
54181
42404
38338
38139
34488
20522
20156
6575
4312
1464
92
(18)
118809
83025
77903
77423
70122
66897
65925
60864
42451
38403
38168
34471
32202
20512
6574
4310
1464
92
(18)
151219
83059
77817
69881
65891
60483
59146
55728
42405
38339
38148
34482
20530
20146
6575
4310
1458
92
(19)
102442
83031
77848
69042
67238
65919
61815
55744
48871
42408
38399
38197
34471
20177
18858
6574
4307
1464
92
(10)
275099
270557
134255
106144
82790
61319
44542
15889
13970
12667
(9)
417579
178689
104525
90421
84906
61340
44540
13970
12981
(12)
170027
140402
95963
83665
66973
52626
39693
36831
35021
31513
16861
7504
(20)
194386
129986
103753
85880
85054
82458
74101
69990
69761
59420
51307
37608
30538
27516
19734
12016
7656
6204
4676
1954
(13)
280238
276899
104507
87606
84555
80516
78218
68145
57429
31973
29568
25841
6208
(13)
271641
267550
104507
87602
84592
80516
78215
68145
57420
31982
29583
25845
6210
(52)
NA
(14)
189915
66946
57955
53997
42689
42327
41106
24005
19186
14502
5191
3264
2090
1222
(14)
300511
267695
235887
135890
93804
63146
48979
47793
44980
41295
38648
21198
18731
76
(27)
78826
75015
73881
67759
64021
51922
41526
32466
32184
30398
29603
29495
29165
28538
28455
24423
23081
22154
12605
11075
10270
5973
5082
4604
2434
1028
411
(27)
75411
75084
73725
69897
67787
51896
41525
32465
32184
30387
29494
29149
28575
28557
28537
24417
23082
22162
12605
11075
10270
5973
4605
4440
2434
1029
411
(27)
75402
75080
73696
67761
64016
51909
41526
32465
32184
30388
29491
29144
28559
28535
28462
24427
23082
22160
12605
11075
10270
5973
4605
4399
2434
1029
411
(27)
78826
75014
73881
67754
64021
51925
41526
32466
32183
30398
29602
29495
29164
28537
28443
24423
23080
22154
12605
11077
10270
5973
5079
4604
2434
1028
411
(18)
163477
136374
123716
122771
80555
71671
49893
49090
46716
36252
28224
18871
11058
7931
6695
4636
3898
2051
(25)
114845
88217
71238
69619
66750
52716
51921
50461
48416
44456
34963
27300
24179
21299
20751
14642
8496
7468
4046
2608
2331
2045
1792
1647
397
(24)
184033
75747
73599
71663
71615
65620
52712
48417
45952
45331
34962
24176
20747
9567
8494
7500
5073
3976
2595
2331
2046
1792
1648
400
(25)
101300
62789
58289
51805
49721
48208
43857
39598
33850
29917
28019
27493
24577
21082
19870
18939
13767
12168
9546
4010
3412
3201
2040
1548
264
(24)
88143
60948
59607
58682
53936
46738
45285
43887
39601
29905
29558
28017
25471
25395
20421
17841
16086
13512
12503
11634
9546
3412
2039
264
(94)
NA
(32)
232436
45205
42876
34902
29508
25866
22866
20898
20096
19490
19187
17417
17099
16266
16218
14837
10962
7845
6556
5345
4915
4175
3116
2226
2049
1988
1877
1732
1178
524
465
108
All data are shown as number of nucleotides.
NA: simulation is not available


1. Mycoplasma agalactiae
2. Mycoplasma agalactiae PG2
3. Mycoplasma arthritidis 158L3-1
4. Mycoplasma bovis HB0801
5. Mycoplasma bovis Hubei-1
6. Mycoplasma bovis PG45
7. Mycoplasma capricolum subsp. capricolum ATCC 27343
8. Mycoplasma conjunctivae
9. Mycoplasma crocodyli MP145
10. Mycoplasma cynos C142
11. Mycoplasma fermentans JER
12. Mycoplasma fermentans M64
13. Mycoplasma fermentans PG18 DNA, nearly
14. Mycoplasma gallisepticum CA06_2006.052-5-2P
15. Mycoplasma gallisepticum NC06_2006.080-5-2P
16. Mycoplasma gallisepticum NC08_2008.031-4-3P
17. Mycoplasma gallisepticum NC95_13295-2-2P
18. Mycoplasma gallisepticum NC96_1596-4-2P
19. Mycoplasma gallisepticum NY01_2001.047-5-1P
20. Mycoplasma gallisepticum R
21. Mycoplasma gallisepticum S6
22. Mycoplasma gallisepticum VA94_7994-1-7P
23. Mycoplasma gallisepticum WI01_2001.043-13-2P
24. Mycoplasma gallisepticum str. F
25. Mycoplasma gallisepticum str. R(high)
26. Mycoplasma genitalium
27. Mycoplasma genitalium M2288
28. Mycoplasma genitalium M2321
29. Mycoplasma genitalium M6282
30. Mycoplasma genitalium M6320
31. Mycoplasma haemocanis str. Illinois
32. Mycoplasma haemofelis Ohio2
33. Mycoplasma haemofelis str. Langford 1
34. Mycoplasma hominis ATCC 23114
35. Mycoplasma hyopneumoniae 168
36. Mycoplasma hyopneumoniae 168-L
37. Mycoplasma hyopneumoniae 232
38. Mycoplasma hyopneumoniae 7422
39. Mycoplasma hyopneumoniae 7448
40. Mycoplasma hyopneumoniae J
41. Mycoplasma hyorhinis DBS 1050
42. Mycoplasma hyorhinis GDL-1
43. Mycoplasma hyorhinis HUB-1
44. Mycoplasma hyorhinis MCLD
45. Mycoplasma hyorhinis SK76
46. Mycoplasma leachii 99/014/6
47. Mycoplasma leachii PG50
48. Mycoplasma mobile 163K
49. Mycoplasma mycoides subsp. capri LC str. 95010
50. Mycoplasma mycoides subsp. mycoides SC
51. Mycoplasma mycoides subsp. mycoides SC str. Gladysdale MU clone SC5
52. Mycoplasma ovis str. Michigan
53. Mycoplasma parvum str. Indiana
54. Mycoplasma penetrans HF-2
55. Mycoplasma pneumoniae
56. Mycoplasma pneumoniae 309
57. Mycoplasma pneumoniae FH
58. Mycoplasma pneumoniae M129-B7
59. Mycoplasma pulmonis
60. Mycoplasma putrefaciens KS1
61. Mycoplasma putrefaciens Mput9231
62. Mycoplasma suis KI_3806
63. Mycoplasma suis str. Illinois
64. Mycoplasma synoviae 53
65. Mycoplasma wenyonii str. Massachusetts