In silico restriction map and PFGE


Genome: Mycoplasma pneumoniae 309
Length of genome: 817176 bp
Restriction Enzyme: AarI
Recognition sequence: CACCTGCNNNN'NNNN_
Number of Cleaves: 50

Simulate PFGE for all Mycoplasma
info



Cleavage PositionLength of sequenceLength of sequence (sorted)PFGE
7446
17044
24579
31753
86302
97054
101193
106211
141830
150207
155373
174326
191562
224076
226338
226836
245161
246275
266735
277099
292195
294058
296092
307843
331445
349694
407719
411440
417434
432028
432949
442304
455756
470297
514868
517844
517934
518789
535480
548360
567338
579848
586486
653337
668892
736393
745088
747622
768993
817175
7447
9598
7535
7174
54549
10752
4139
5018
35619
8377
5166
18953
17236
32514
2262
498
18325
1114
20460
10364
15096
1863
2034
11751
23602
18249
58025
3721
5994
14594
921
9355
13452
14541
44571
2976
90
855
16691
12880
18978
12510
6638
66851
15555
67501
8695
2534
21371
48182

67501
66851
58025
54549
48182
44571
35619
32514
23602
21371
20460
18978
18953
18325
18249
17236
16691
15555
15096
14594
14541
13452
12880
12510
11751
10752
10364
9598
9355
8695
8377
7535
7447
7174
6638
5994
5166
5018
4139
3721
2976
2534
2262
2034
1863
1114
921
855
498
90


























































All data are shown as number of nucleotides.