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Restriction digest of Mycoplasma pneumoniae 309

This service shows theoretical number of cuts generated by commercial restriction enzymes.
When the number of cuts is less than 50, PFGE simulation is available by following the link.

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Restriction enzymesSequenceFragments
AarICACCTGCNNNN'NNNN_50
AasI,DrdI,DseDIGACNN_NN'NNGTC109
AatI,Eco147I,PceI,SseBI,StuIAGG'CCT112
AatIIG_ACGT'C21
AbsICC'TCGA_GG1
Acc16I,AviII,FspI,NsbITGC'GCA154
Acc36I,BfuAI,BspMI,BveIACCTGCNNNN'NNNN_176
Acc65I,Asp718IG'GTAC_C209
AccB1I,BanI,BshNI,BspT107IG'GYRC_C486
AccB7I,BasI,PflMI,Van91ICCAN_NNN'NTGG248
AccBSI,BsrBI,MbiICCG'CTC157
AccI,FblI,XmiIGT'MK_AC215
AccII,Bsh1236I,BspFNI,BstFNI,BstUI,MvnICG'CG1271
AccIII,Aor13HI,BlfI,BseAI,Bsp13I,BspEI,Kpn2I,MroIT'CCGG_A66
AciI,BspACI,SsiIC'CG_C or G'CG_G3337
AclI,Psp1406IAA'CG_TT300
AclWI,AlwI,BspPIGGATCNNNN'N_993
AcoIY'CCGG_R312
AcsI,ApoI,XapIR'AATT_Y1596
AcuI,Eco57ICTGAAGNNNNNNNNNNNNNN_NN'322
AcvI,BbrPI,Eco72I,PmaCI,PmlI,PspCICAC'GTG67
AcyI,BsaHI,BssNI,BstACI,Hin1I,Hsp92IGR'CG_YC111
AdeI,DraIIICAC_NNN'GTG119
AfaI,RsaIGT'AC2481
AfeI,Aor51HI,Eco47IIIAGC'GCT301
AfiI,Bsc4I,BseLI,BsiYI,BslICCNN_NNN'NNGG1723
AflII,BfrI,BspTI,Bst98I,BstAFI,MspCI,Vha464IC'TTAA_G181
AflIIIA'CRYG_T258
AgeI,AsiGI,BshTI,CspAI,PinAIA'CCGG_T177
AhdI,AspEI,BmeRI,DriI,Eam1105I,EclHKIGACNN_N'NNGTC56
AhlI,BcuI,SpeIA'CTAG_T277
AjiI,BmgBI,BtrICAC'GTC80
AjnI,EcoRII,Psp6I,PspGI'CCWGG_1596
AjuI_NNNNN'NNNNNNNGAANNNNNNNTTGGNNNNNN_NNNNN_'
Produces double-strand cuts		179
AleI,OliICACNN'NNGTG138
AlfI_NN'NNNNNNNNNNCGANNNNNNTGCNNNNNNNNNN_NN'
Produces double-strand cuts		226
AloI_NNNNN'NNNNNNNGAACNNNNNNTCCNNNNNNN_NNNNN'
Produces double-strand cuts		60
AluI,AluBIAG'CT4192
Alw21I,BsiHKAI,Bbv12IG_WGCW'C402
Alw26I,BcoDI,BsmAI,BstMAIGTCTCN'NNNN_302
Alw44I,ApaLI,VneIG'TGCA_C126
AlwNI,CaiI,PstNICAG_NNN'CTG122
Ama87I,AvaI,BmeT110I,BsiHKCI,BsoBI,Eco88IC'YCGR_G196
ApaIG_GGCC'C15
ApeKI,TseIG'CWG_C1175
AscI,PalAI,SgsIGG'CGCG_CC7
AseI,PshBI,VspIAT'TA_AT608
AsiSI,RgaI,SfaAI,SgfIGCG_AT'CGC8
Asp700I,MroXI,PdmI,XmnIGAANN'NNTTC271
AspA2I,AvrII,BlnI,XmaJIC'CTAG_G29
AspI,PflFI,PsyI,Tth111IGACN'N_NGTC90
AspLEI,BstHHI,CfoI,HhaIG_CG'C1973
AspS9I,BmgT120I,Cfr13I,PspPI,Sau96IG'GNC_C686
AssI,BmcAI,ScaI,ZrmIAGT'ACT246
AsuII,Bpu14I,Bsp119I,BspT104I,BstBI,Csp45I,NspV,SfuITT'CG_AA138
AsuC2I,BcnI,BpuMI,NciICC'S_GG638
AsuHPI,HphIGGTGANNNNNNN_N'1781
AsuNHI,BspOI,NheIG'CTAG_C196
AvaII,Bme18I,Eco47I,SinI,VpaK11BIG'GWC_C427
AxyI,Bse21I,Bsu36I,Eco81ICC'TNA_GG79
BaeI_NNNNN'NNNNNNNNNNACNNNNGTAYCNNNNNNN_NNNNN'
Produces double-strand cuts		128
BalI,MlsI,MluNI,MscI,Msp20ITGG'CCA167
BamHIG'GATC_C87
BanII,Eco24I,EcoT38I,FriOIG_RGCY'C202
BanIII,Bsa29I,BseCI,BshVI,BspDI,BspXI,Bsu15I,BsuTUI,ClaIAT'CG_AT120
BarI_NNNNN'NNNNNNNGAAGNNNNNNTACNNNNNNN_NNNNN'
Produces double-strand cuts		80
BauIC'ACGA_G58
BbeI,PluTIG_GCGC'C39
BbsI,BpiI,BpuAI,BstV2IGAAGACNN'NNNN_273
BbuI,PaeI,SphIG_CATG'C38
BbvCICC'TCA_GC or GC'TGA_GG45
BbvI,BseXI,BstV1I,Lsp1109IGCAGCNNNNNNNN'NNNN_1145
BccICCATCNNNN'N_1289
BceAIACGGCNNNNNNNNNNNN'NN_708
BcgI_NN'NNNNNNNNNNCGANNNNNNTGCNNNNNNNNNN_NN'
Produces double-strand cuts		226
BciVI,BfuI,BsuIGTATCCNNNNN_N'151
BclI,FbaI,Ksp22IT'GATC_A325
BdaI_NN'NNNNNNNNNNTGANNNNNNTCANNNNNNNNNN_NN'
Produces double-strand cuts		318
BfaI,FspBI,MaeI,XspIC'TA_G2009
BfiI,BmrI,BmuIACTGGGNNNN_N'324
BfmI,BpcI,BstSFI,SfcIC'TRYA_G327
BfuCI,Bsp143I,BssMI,BstMBI,DpnII,Kzo9I,MboI,NdeII,Sau3AI'GATC_5016
BglIGCCN_NNN'NGGC72
BglIIA'GATC_T171
BisI,BlsI,Fnu4HI,Fsp4HI,GluI,ItaI,SatIGC'N_GC1720
BlpI,Bpu1102I,Bsp1720I,CelIIGC'TNA_GC165
Bme1390I,MspR9I,ScrFICC'N_GG1436
326
BmiI,BspLI,NlaIV,PspN4IGGN'NCC1052
BmrFI,BssKI,BstSCI,StyD4I'CCNGG_2872
BmtIG_CTAG'C196
BoxI,PshAI,BstPAIGACNN'NNGTC53
BplI_NNNNN'NNNNNNNNGAGNNNNNCTCNNNNNNNN_NNNNN'
Produces double-strand cuts		37
BpmI,GsuICTGGAGNNNNNNNNNNNNNN_NN'215
Bpu10ICC'TNA_GC289
BpuEICTTGAGNNNNNNNNNNNNNN_NN'384
BpvUI,MvrI,PvuI,Ple19ICG_AT'CG57
BsaAI,BstBAI,Ppu21IYAC'GTR265
BsaBI,Bse8I,BseJI,MamIGATNN'NNATC195
BsaI,Bso31I,BspTNI,Eco31IGGTCTCN'NNNN_87
BsaJI,BseDI,BssECIC'CNNG_G1517
BsaMI,BsmI,Mva1269I,PctIGAATG_CN'174
BsaWIW'CCGG_W494
BsaXI_NNN'NNNNNNNNNACNNNNNCTCCNNNNNNN_NNN'
Produces double-strand cuts		161
Bse118I,BsrFI,BssAI,Cfr10IR'CCGG_Y362
Bse1I,BseNI,BsrI,BsrSIACTG_GN'1799
Bse3DI,BseMI,BsrDIGCAATG_NN'479
BciT130I,BseBI,BstNI,BstOI,Bst2UI,MvaICC'W_GG798
BseGI,BstF5IGGATG_NN'1081
BseMIICTCAGNNNNNNNN_NN'600
BsePI,BssHII,PauI,PteIG'CGCG_C110
BseRIGAGGAGNNNNNNNN_NN'122
BseX3I,BstZI,EagI,EclXI,Eco52IC'GGCC_G41
BseYIC'CCAG_C245
BsgIGTGCAGNNNNNNNNNNNNNN_NN'250
Bsh1285I,BsiEI,BstMCICG_RY'CG206
BshFI,BsnI,BspANI,BsuRI,HaeIII,PhoIGG'CC1159
BsiSI,HapII,HpaII,MspIC'CG_G1398
BsiWI,Pfl23II,PspLIC'GTAC_G44
BslFI,FaqIGGGACNNNNNNNNNN'NNNN_486
BsmBI,Esp3ICGTCTCN'NNNN_27
BsmFIGGGACNNNNNNNNNN'NNNN_486
Bsp120I,PspOMIG'GGCC_C15
Bsp1286I,MhlI,SduIG_DGCH'C698
Bsp1407I,BsrGI,BstAUI,SspBIT'GTAC_A111
Bsp19I,NcoIC'CATG_G131
Bsp68I,BtuMI,NruI,RruITCG'CGA57
BspCNICTCAGNNNNNNN_NN'600
BspHI,CciI,PagI,RcaIT'CATG_A134
BspLU11I,PciI,PscIA'CATG_T76
BspMAI,PstIC_TGCA'G82
BspQI,LguI,PciSI,SapIGCTCTTCN'NNN_78
BssNAI,Bst1107I,BstZ17IGTA'TAC38
BssSI,Bst2BIC'ACGA_G or C'TCGT_G110
BssT1I,StyI,Eco130I,EcoT14I,ErhIC'CWWG_G646
Bst4CI,HpyCH4III,TaaIAC_N'GT3666
Bst6I,Eam1104I,EarI,Ksp632ICTCTTCN'NNN_371
BstAPIGCAN_NNN'NTGC167
BstC8I,Cac8IGCN'NGC1378
BstDEI,DdeI,HpyF3IC'TNA_G2391
BstDSI,BtgIC'CRYG_G421
BstEII,BstPI,Eco91I,EcoO65I,PspEIG'GTNAC_C197
BstENI,EcoNI,XagICCTNN'N_NNAGG112
BfoI,BstH2I,HaeIIR_GCGC'Y590
BstKTIG_AT'C2508
BstMWI,MwoIGCNN_NNN'NNGC2027
BstNSI,NspI,XceIR_CATG'Y247
BstSNI,Eco105I,SnaBITAC'GTA68
BstX2I,BstYI,MflI,PsuI,XhoIIR'GATC_Y550
BstXICCAN_NNNN'NTGG275
BtgZIGCGATGNNNNNNNNNN'NNNN_191
BtsIGCAGTG_NN'399
CciNI,NotIGC'GGCC_GC2
Cfr42I,KspI,SacII,Sfr303I,SgrBI,SstIICC_GC'GG67
Cfr9I,TspMI,XmaI,XmaCIC'CCGG_G76
CfrI,EaeIY'GGCC_R344
CpoI,CspI,RsrII,Rsr2ICG'GWC_CG23
Csp6I,CviQI,RsaNIG'TA_C2481
CspCI_NN'NNNNNNNNNNNCAANNNNNGTGGNNNNNNNNNN_NN'
Produces double-strand cuts		147
CviAII,FaeI,Hin1II,Hsp92II,NlaIII_CATG'1547
CviJI,CviKI-1RG'CY9101
DinI,Mly113I,NarI,SspDIGG'CG_CC39
DpnI,MalIGA'TC2508
DraITTT'AAA1459
EciIGGCGGANNNNNNNNN_NN'144
Ecl136II,Eco53kI,EcoICRIGAG'CTC91
Eco32I,EcoRVGAT'ATC67
Eco57MICTGRAGNNNNNNNNNNNNNN_NN'530
EcoO109I,DraIIRG'GNC_CY109
EcoP15ICAGCAGNNNNNNNNNNNNNNNNNNNNNNNNN'NN_351
EcoRIG'AATT_C158
EcoT22I,Mph1103I,NsiI,Zsp2IA_TGCA'T79
EgeI,EheI,SfoIGGC'GCC39
FalI_NNNNN'NNNNNNNNAAGNNNNNCTTNNNNNNNN_NNNNN'
Produces double-strand cuts		516
FatI'CATG_3094
FauI,SmuICCCGCNNNN'NN_688
FauNDI,NdeICA'TA_TG56
FokI,BtsCIGGATGNNNNNNNNN'NNNN_1064
FseI,RigIGG_CCGG'CC1
FspAIRTGC'GCAY20
GlaIGC'GC1973
HgaI,CseIGACGCNNNNN'NNNNN_375
Hin4I_NNNNN'NNNNNNNNGAYNNNNNVTCNNNNNNNN_NNNNN'
Produces double-strand cuts		458
Hin6I,HinP1I,HspAIG'CG_C1973
HincII,HindIIGTY'RAC996
HindIIIA'AGCT_T454
HinfIG'ANT_C1598
HpaI,KspAIGTT'AAC447
Hpy188ITC_N'GA1180
Hpy188IIITC'NN_GA1755
Hpy166II,Hpy8IGTN'NAC2644
Hpy99I_CGWCG'220
HpyAVGCTCTTCN'NNN_78
HpyCH4IV,HpySE526I,MaeIIA'CG_T1447
HpyCH4VTG'CA2582
HpyF10VIGCNN_NNN'NNGC2027
KasIG'GCGC_C39
KpnIG_GTAC'C209
BmsI,LweI,SfaNIGCATCNNNNN'NNNN_1046
CsiI,MabI,SexAIA'CCWGG_T158
MaeIII'GTNAC_5300
MboIIGAAGANNNNNNN_N'1809
MfeI,MunIC'AATT_G463
MluIA'CGCG_T66
MlyI,SchIGAGTCNNNNN'504
MmeITCCRACNNNNNNNNNNNNNNNNNN_NN'671
MnlICCTCNNNNNN_N'2718
MreICG'CCGG_CG2
KroI,MroNI,NgoMIVG'CCGG_C35
MseI,SaqAI,Tru1I,Tru9IT'TA_A11045
MslI,RseI,SmiMICAYNN'NNRTG603
MspA1ICMG'CKG542
MssI,PmeIGTTT'AAAC63
NaeI,PdiIGCC'GGC35
NmeAIIIGCCGAGNNNNNNNNNNNNNNNNNNN_NN'84
NmuCI,TseFI,Tsp45I'GTSAC_1816
PacITTA_AT'TAA170
PaeR7I,Sfr274I,SlaI,StrI,TliI,XhoIC'TCGA_G26
PasICC'CWG_GG44
PfeI,TfiIG'AWT_C1093
PfoIT'CCNGG_A119
PleI,PpsIGAGTCNNNN'N_504
PpiI_NNNNN'NNNNNNNGAACNNNNNCTCNNNNNNNN_NNNNN'
Produces double-strand cuts		48
PpuMI,Psp5II,PspPPIRG'GWC_CY75
AanI,PsiITTA'TAA218
Psp124BI,SacI,SstIG_AGCT'C91
PspXIVC'TCGA_GB20
PsrI_NNNNN'NNNNNNNGAACNNNNNNTACNNNNNNN_NNNNN'
Produces double-strand cuts		74
PvuIICAG'CTG208
SalIG'TCGA_C69
13
SbfI,SdaI,Sse8387ICC_TGCA'GG1
SetI_ASST'11798
SfiIGGCCN_NNN'NGGCC1
SgrAICR'CCGG_YG24
SgrDICG'TCGA_CG0
SmaICCC'GGG76
SmiI,SwaIATTT'AAAT60
SmlI,SmoIC'TYRA_G594
SrfIGCCC'GGGC2
MluCI,Sse9I,TasI,Tsp509I,TspEI'AATT_15734
SspIAAT'ATT300
TaiI_ACGT'1447
TaqIT'CG_A1669
TaqIIGACCGANNNNNNNNN_NN' or CACCCANNNNNNNNN_NN'489
TatIW'GTAC_W805
TauIG_CSG'C570
TsoITARCCANNNNNNNNN_NN'1277
TspDTIATGAANNNNNNNNN_NN'2232
TspGWIACGGANNNNNNNNN_NN'759
TscAI,TspRI_NNCASTGNN'1626
TstI_NNNNN'NNNNNNNNCACNNNNNNTCCNNNNNNN_NNNNN'
Produces double-strand cuts		191
XbaIT'CTAG_A89
XcmICCANNNN_N'NNNNTGG269
ZraIGAC'GTC21
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