In silico PCR amplification with users sequences


Primer 11    5'- -3' C
Primer 21    5'- -3' C

Select sequence

Allow mismatches, but in nucleotides in 3' end

Maximum lenght of bands
  nucleotides

1 Degenerated nucleotides are allowed; A+T+G+C must be 10 or more.


This tool may be used with several sequences uploaded in fasta format in an unique file.
Do not use the file in the other services but PCR, PCR-RFLP and AFLP-PCR amplification (aberrant results will be obtained).