Simulation is performed in three steps
- The DNA sequence to be used as a probe is amplified by PCR
- Endonuclease for complete genome restriction is selected
- Simulation is performed: genome is virtually restricted,
and fragments containing the probe matching sequences are shown
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Selection of probe
 By PCR amplification
Primer 11 5'- -3'
C
Primer 21 5'- -3'
C
Allow
mismatches, but in 3' end
Maximum lenght of bands
nucleotides
Sequence
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