In silico Double Digestion
Fingerprinting
(DDF)
This simulation tool for Double Digestion Fingerprinting
technique allows AFLP-PCR and SRF techniques to be simulated, and
also
novel aprouches. We
have included a few examples to show the us of this
tool.
 Simulation of AFLP-PCR
experiment
Simulation of Subtracted
Restriction Fingerprinting (SRF) experiment
Simulation of AFLP-PCR
like experiment
when using one non-palindromic endonuclease
Simulation of AFLP-PCR
like experiment
when using a non-palindromic endonuclease
with degenerated (N)
nucleotides
Simulation of AFLP-PCR
like experiment
when using only one endonuclease
Simulation
of Double Digestion Fingerprinting experiment and detection of bands
with labelled
adaptors
Have you tried a different approuch not listed above? Please let us know.
Frequency of recognition site for commercial endonucleases in sequenced
bacterial genomes may be
checked here.
|